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Academia Open

Clinical Research

Vol 9 No 2 (2024): December

Identification Methods of Helicobacter Pylori Bacteria
Metode Identifikasi Bakteri Helicobacter Pylori



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.9.2024.9846
Published
August 22, 2024

Abstract

Background: Helicobacter pylori is a well-known carcinogenic pathogen associated with gastrointestinal diseases, often infecting individuals during childhood and persisting asymptomatically for years. Specific Background: The failure to diagnose H. pylori early can result in severe health consequences, making timely and accurate diagnosis critical to preventing its spread. Knowledge Gap: Diagnostic methods' effectiveness depends on patient age, economic status, and operator skill, with some tests being expensive and influenced by protocol adherence. Aims: The study evaluates the efficacy of various diagnostic methods for H. pylori, focusing on the accuracy, reliability, and practicality of invasive and non-invasive techniques. Results: Invasive methods like histology, endoscopy, culture, rapid urease test, and polymerase chain reaction (PCR) provide direct detection of the bacterium, while non-invasive methods such as the urea breath test, stool antigen assay, and serology offer indirect detection without requiring endoscopy. PCR, when conducted with appropriate primers and gene targets, remains the most accurate diagnostic tool. Novelty: The study emphasizes the superior diagnostic value of PCR due to its precision, despite challenges related to operator skill and procedural compliance. Implications: The study emphasizes the significance of selecting suitable diagnostic methods based on patient-specific factors and the need for skilled execution for accurate results in managing and treating H. pylori infections.

Highlights:

 

  1. PCR Accuracy: Most reliable H. pylori test with correct primers and targets.
  2. Operator Skill: Diagnosis depends on skilled execution and procedural adherence.
  3. Test Selection: Choose based on patient age, economic status, and method availability.

 

Keywords: Helicobacter pylori, diagnosis, gastrointestinal diseases, PCR, invasive tests

References

  1. . S. Flores-Treviño, S. Mendoza-Olazarán, P. Bocanegra-Ibarias, H. J. Maldonado-Garza, and E. Garza-González, "Helicobacter pylori Drug Resistance: Therapy Changes and Challenges," Expert Review of Gastroenterology & Hepatology, vol. 12, pp. 819-827, 2018, doi: 10.1080/17474124.2018.1496017.
  2. . S. Hirukawa, H. Sagara, S. Kaneto, T. Kondo, K. Kiga, and T. Sanada, et al., "Characterization of Morphological Conversion of Helicobacter pylori Under Anaerobic Conditions," Microbiology and Immunology, vol. 62, pp. 221-228, 2018, doi: 10.1111/1348-0421.12582.
  3. . M. Liu, H. Gao, J. Miao, Z. Zhang, L. Zheng, F. Li, et al., "Helicobacter pylori Infection in Humans and Phytotherapy, Probiotics, and Emerging Therapeutic Interventions: A Review," Frontiers in Microbiology, vol. 14, art. 1330029, 2024, doi: 10.3389/fmicb.2023.1330029.
  4. . P. Isaacson and D. H. Wright, "Malignant Lymphoma of Mucosa-Associated Lymphoid Tissue: A Distinctive Type of B-Cell Lymphoma," Cancer, vol. 52, pp. 1410-1416, 1983, doi: 10.1002/1097-0142(19831015)52:8<1410::AID-CNCR2820520813>3.0.CO;2-3.
  5. . H. C. Sharndama and I. E. Mba, "Helicobacter pylori: An Up-to-Date Overview on the Virulence and Pathogenesis Mechanisms," Brazilian Journal of Microbiology, vol. 53, pp. 33-50, 2022, doi: 10.1007/s42770-021-00675-0.
  6. . A. Elbehiry, E. Marzouk, M. Aldubaib, A. Abalkhail, S. Anagreyyah, N. Anajirih, et al., "Helicobacter pylori Infection: Current Status and Future Prospects on Diagnostic, Therapeutic and Control Challenges," Antibiotics, vol. 12, art. 191, 2023, doi: 10.3390/antibiotics12020191.
  7. . N. L. Jones et al., "Joint ESPGHAN/NASPGHAN Guidelines for the Management of Helicobacter pylori in Children and Adolescents," Journal of Pediatric Gastroenterology and Nutrition, vol. 64, pp. 991-1003, 2017.
  8. . L. A. Spee, M. B. Madderom, M. Pijpers, Y. van Leeuwen, and M. Y. Berger, "Association Between Helicobacter pylori and Gastrointestinal Symptoms in Children," Pediatrics, vol. 125, pp. e651-e669, 2010.
  9. . G. Khoder, J. S. Muhammad, I. Mahmoud, S. S. M. Soliman, and C. Burucoa, "Prevalence of Helicobacter pylori and Its Associated Factors Among Healthy Asymptomatic Residents in the United Arab Emirates," Pathogens, vol. 8, art. 44, 2019, doi: 10.3390/pathogens8020044.
  10. . S. Kayali, M. Manfredi, F. Gaiani, L. Bianchi, B. Bizzarri, G. Leandro, et al., "Helicobacter pylori, Transmission Routes and Recurrence of Infection: State of the Art," Acta Biomedica, vol. 89, pp. 72-76, 2018, doi: 10.23750/abm.v89i8-S.7947.
  11. . E. Garza-González, G. I. Perez-Perez, H. J. Maldonado-Garza, and F. J. Bosques-Padilla, "A Review of Helicobacter pylori Diagnosis, Treatment, and Methods to Detect Eradication," World Journal of Gastroenterology, vol. 20, no. 6, pp. 1438-1449, 2014.
  12. . P. Malfertheiner, M. C. Camargo, E. El-Omar, J. Liou, R. Peek, C. Schulz, S. I. Smith, and S. Suerbaum, "Helicobacter pylori Infection: Current Status and Future Prospects on Diagnostic, Therapeutic and Control Challenges," Nature Reviews Disease Primers, vol. 9, art. 19, 2023, doi: 10.1038/s41572-023-00431-8.
  13. . J. Y. Lee and N. Kim, "Diagnosis of Helicobacter pylori by Invasive Test: Histology," Annals of Translational Medicine, vol. 3, no. 1, art. 10, 2015, doi: 10.3978/j.issn.2305-5839.2014.11.03.
  14. . R. A. Hussein, M. T. S. Al-Ouqaili, and H. M. Majeed, "Detection of Helicobacter pylori Infection by Invasive and Non-Invasive Techniques in Patients With Gastrointestinal Diseases From Iraq: A Validation Study," PLOS ONE, vol. 16, art. e0256393, 2021, doi: 10.1371/journal.pone.0256393.
  15. . V. Ricci, P. Monzo, T. Gonzalez, A. Doye, A. Oldani, P. Gounon, M. Cormont, and P. Boquet, "Early Endosomes Associated With Dynamic F-Actin Structures Are Required for Late Trafficking of VacA Toxin," Journal of Cell Biology, vol. 177, pp. 343-354, 2007.
  16. . Y. K. Wang, F. C. Kuo, C. J. Liu, M. C. Wu, H. Y. Shih, S. S. Wang, et al., "Diagnosis of Helicobacter pylori Infection: Current Options and Developments," World Journal of Gastroenterology, vol. 21, no. 40, pp. 11221-11235, 2015, doi: 10.3748/wjg.v21.i40.11221.
  17. . J. H. Lee, Y. S. Park, K. S. Choi, D. H. Kim, K. D. Choi, H. J. Song, et al., "Optimal Biopsy Site for Helicobacter pylori Detection During Endoscopic Mucosectomy in Patients With Extensive Gastric Atrophy," Helicobacter, vol. 17, no. 6, pp. 405-410, 2012.
  18. . P. Malfertheiner, F. Megraud, C. A. O'Morain, J. Atherton, A. T. Axon, and F. Bazzoli, et al., "Management of Helicobacter pylori Infection—The Maastricht IV/Florence Consensus Report," Gut, vol. 61, no. 5, pp. 646-664, 2012.
  19. . H. M. Hadi, "Helicobacter pylori in Pediatric Patients, Serovalence Association With Clinical Symptoms," College of Medicine, University of Baghdad, Iraq, 2005, pp. 9-22.
  20. . Z. K. Sabah, "Helicobacter pylori IgG Antibodies in Iraqi Uremic Patients in Association With Pepsinogens and Human Leukocyte Antigens Class I and II," College of Medicine, University of Baghdad, Iraq, 2011, pp. 3-26.
  21. . H. Korkmaz, D. Findik, C. Ugurluoglu, and Y. Terzi, "Reliability of Stool Antigen Tests: Investigation of the Diagnostic Value of a New Immune Chromatographic Helicobacter pylori Approach in Dyspeptic Patients," Asian Pacific Journal of Cancer Prevention, vol. 16, no. 2, pp. 657-660, 2015, doi: 10.7314/APJCP.2015.16.2.657.
  22. . D. Vaira, N. Vakil, L. Gatta, C. Ricci, F. Perna, I. Saracino, G. Fiorini, and J. Holton, "Accuracy of a New Ultrafast Rapid Urease Test to Diagnose Helicobacter pylori Infection in 1000 Consecutive Dyspeptic Patients," Alimentary Pharmacology & Therapeutics, vol. 31, no. 2, pp. 331-338, 2010, doi: 10.1111/j.1365-2036.2009.04196.x.
  23. . R. D. M. Al-Hilali and H. D. K. Al-Mozan, "Molecular Detection and Probiotic Treatment for Bacteria That Cause Diarrhea," Advancements in Life Sciences, vol. 10, no. 1, pp. 118-123, 2023.
  24. . E. Rimbara, M. Sasatsu, and D. Y. Graham, "PCR Detection of Helicobacter pylori in Clinical Samples," Methods in Molecular Biology, vol. 943, pp. 279-287, 2013.
  25. . H. D. K. Al-Mozan, "Review: Gene of 16S rRNA is the Gold Standard to Diagnose Bacteria," World Journal of Current Medical and Pharmaceutical Research, vol. 5, no. 4, pp. 139-148, 2023.
  26. . E. N. Kalali, X. Wang, and D. Y. Wang, "Functionalized Layered Double Hydroxide-Based Epoxy Nanocomposites With Improved Flame Retardancy and Mechanical Properties," Journal of Materials Chemistry A, vol. 3, no. 13, pp. 6819-6826, 2015.
  27. . A. B. M. Carlos, C. V. de Oliveira, M. A. M. Rodrigues, and V. E. Costa, "Is Breath Testing Without 13C-Labelled External Urea Able to Detect Helicobacter pylori Infection?," Analytical and Bioanalytical Chemistry, vol. 411, no. 22, pp. 5641-5645, 2019.
  28. . P. Malfertheiner, F. Megraud, C. A. O'Morain, J. P. Gisbert, E. J. Kuipers, A. T. Axon, et al., "Management of Helicobacter pylori Infection—The Maastricht V/Florence Consensus Report," Gut, vol. 66, no. 1, pp. 6-30, 2017.
  29. . J. P. Gisbert and A. G. McNicholl, "Optimization Strategies Aimed to Increase the Efficacy of Helicobacter pylori Eradication Therapies," Helicobacter, vol. 22, no. 4, art. e12392, 2017.
  30. . J. Guarner, N. Kalach, Y. Elitsur, and S. Koletzko, "Helicobacter pylori Diagnostic Tests in Children: Review of the Literature From 1999 to 2009," European Journal of Pediatrics, vol. 169, no. 1, pp. 15-25, 2010.
  31. . P. Sabbagh, M. Mohammadnia-Afrouzi, and M. Javanian, "Diagnostic Methods for Helicobacter pylori Infection: Ideals, Options, and Limitations," European Journal of Clinical Microbiology & Infectious Diseases, vol. 38, pp. 55-66, 2019.

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