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Academia Open

Clinical Research

Vol 9 No 2 (2024): December

Cladophora spp. Extracts Show Remarkable Antibacterial Potential Against Pseudomonas aeruginosa
Ekstrak Cladophora spp. Menunjukkan Potensi Antibakteri yang Luar Biasa Terhadap Pseudomonas aeruginosa



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.9.2024.9842
Published
August 22, 2024

Abstract

Background: Bacterial resistance, influenced by genetic processes and adaptive strategies, necessitates the discovery of novel antibacterial agents, especially from natural sources. Specific Background: Pseudomonas aeruginosa, a notorious pathogen in urinary tract infections (UTIs), demonstrates considerable resistance to conventional therapies, necessitating alternative therapeutic approaches. Knowledge Gap: Research indicates that while natural sources like Cladophora spp. offer antibacterial agents, their effectiveness in combating P. aeruginosa resistant strains remains underexplored. Aims: This study aims to evaluate the antibacterial potential of Cladophora spp. algae extracts against Pseudomonas aeruginosa isolated from UTIs, utilizing solvent extraction, MIC determination, disc diffusion assays, and GC-MS analysis to identify bioactive compounds. Results: All extracts, including those prepared with water, ethanol, and hexane, demonstrated inhibitory effects on P. aeruginosa. The hexane extract exhibited the most significant activity, with a mean zone of inhibition of 13.0 ± 0.7 mm at a concentration of 50%. GC-MS analysis identified several bioactive compounds potentially responsible for these effects. Novelty: This study is among the first to investigate Cladophora spp. as a source of antibacterial agents specifically targeting P. aeruginosa, providing new insights into the potential of algae-based therapeutics. Implications: Cladophora spp. holds promise as a source of novel antibacterial compounds, with potential for multidrug-resistant infections treatments. Further research is needed for clinical application.

Highlights:

 

  1. Hexane Extract: Most effective against Pseudomonas aeruginosa.
  2. Novel Source: Cladophora spp. shows potential as antibacterial agent.
  3. GC-MS Findings: Identified key bioactive compounds.

 

Keywords: Cladophora spp., Pseudomonas aeruginosa, antibacterial agents, natural extracts, GC-MS analysis

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