Academia Open
Vol 9 No 2 (2024): December
Clinical Research

The Study On Antioxidant And Anti-Inflammatory Effects Of Sodium Copper Chlorophyllin In Male Rats Receiving Indomethacin


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  • Ibrahim H. Muslim*,
  • Nadheerah F. Neamah,
  • Falah H. Shari
Ibrahim H. Muslim
Pharmacology &Toxicology Department, College of Pharmacy, University of Basrah, Basrah, Iraq *
Nadheerah F. Neamah
Pharmacology &Toxicology Department, College of Pharmacy, University of Basrah, Basrah, Iraq
Falah H. Shari
University of Maaqal, Iraq

(*) Corresponding Author
Picture in here are illustration from public domain image or provided by the author, as part of their works
DOI: https://doi.org/10.21070/acopen.9.2024.9223
Published July 7, 2024
Keywords
  • Sodium copper chlorophyllin,
  • antioxidant,
  • anti-inflammatory,
  • indomethacin,
  • oxidative stress
How to Cite
Muslim, I. H., Neamah, N. F., & Shari, F. H. (2024). The Study On Antioxidant And Anti-Inflammatory Effects Of Sodium Copper Chlorophyllin In Male Rats Receiving Indomethacin. Academia Open, 9(2), DOI 10.21070/acopen.9.2024.9223. https://doi.org/10.21070/acopen.9.2024.9223

Copyright (c) 2024 Ibrahim H. Muslim, Nadheerah F. Neamah, Falah H. Shari

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Sodium copper chlorophyllin (SC-C) is widely used for its biological properties and color. This study evaluated SC-C's antioxidative and anti-inflammatory effects in male rats treated with indomethacin. Fifty rats were divided into five groups: control, indomethacin, rabeprazole, and two SC-C dosages (35 mg/kg and 70 mg/kg). Indomethacin reduced serum GPx levels, but rabeprazole and SC-C treatments restored them. MDA levels increased with indomethacin but normalized with other treatments. Indomethacin also lowered IL-10, IL-4, and TNF, which returned to normal in treated groups. Histopathology showed SC-C reversed liver damage from indomethacin. SC-C demonstrates strong antioxidant and anti-inflammatory activities, suggesting its potential as a therapeutic agent.

Highlight:

 

  1. SC-C significantly restores serum GPx levels reduced by indomethacin.
  2. SC-C normalizes MDA, IL-10, IL-4, and TNF levels, countering oxidative stress.
  3. Histopathology shows SC-C reverses liver damage induced by indomethacin.

 

Key wards: Sodium copper chlorophyllin, antioxidant, anti-inflammatory, indomethacin, oxidative stress

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References

  1. Carlini, V., Noonan, D. M., Abdalalem, E., Goletti, D., Sansone, C., Calabrone, L., & Albini, A. (2023). The multifaceted nature of IL-10: regulation, role in immunological homeostasis and its relevance to cancer, COVID-19 and post-COVID conditions. Frontiers in Immunology, 14. doi: 10.3389/fimmu.2023.1161067
  2. Ciciliato, M. P., de Souza, M. C., Tarran, C. M., de Castilho, A. L. T., Vieira, A. J., & Rozza, A. L. (2022). Anti-Inflammatory Effect of Vanillin Protects the Stomach against Ulcer Formation. Pharmaceutics, 14(4). doi: 10.3390/pharmaceutics14040755
  3. Ibtihal J. Naoor, N. F. N. a. J. K. H. (2023). A study of the protective effect of 5 Thiocyanatouracil against paracetamol overdose in rats History of Medicine, 9(2), 1-9. doi: https://doi.org/10.17720/2409-5834.v9.2.2023.001
  4. Lanfer-Marquez, U. M., Barros, R. M. C., & Sinnecker, P. (2005). Antioxidant activity of chlorophylls and their derivatives. Food Research International, 38(8), 885-891. doi: https://doi.org/10.1016/j.foodres.2005.02.012
  5. Lee, S. W., Hong, Y. S., Chun, C. M., Moon, J. D., Kim, S. J., Jung, I. C., . . . Moon, C. K. (2002). Anti-inflammatory effects of IL-4 and IL-10 on human polymorphonuclear leukocytes. J Korean Med Sci, 17(1), 7-14. doi: 10.3346/jkms.2002.17.1.7
  6. Lv, H., Lin, Y., Liu, P., Liang, W., Wei, K., Pu, J., & Zhang, H. (2019). Protective effects and potential underlying mechanisms of sodium copper chlorophyllin against ethanol-induced gastric ulcer in mice. Acta Biochim Biophys Sin (Shanghai), 51(9), 925-933. doi: 10.1093/abbs/gmz083
  7. Neamah, N., Naaeem Khudair, A.-R., & Al-Jadaan, S. A. N. (2020). Antioxidant activity of novel selena-diazole derivative against oxidative stress induced by dipyrone in female rats. Physiology and Pharmacology, 24(3), 202-210. doi: 10.32598/ppj.24.3.60
  8. Neamah, N. F., Naaeem Khudair, A.-R., & Al-Jadaan, S. A. N. (2019). Comparative evaluation of biochemical profiling of 4’, 4”- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) and dipyrone on female rat’s. Journal of Physics: Conference Series, 1279(1), 012033. doi: 10.1088/1742-6596/1279/1/012033
  9. Nissa, C., Kartasurya, M. I., & Rahmawati, B. (2016). Effects of chlorophyll in papaya leaves on superoxide dismutation and blood glucose level of diabetic rats. Makara Journal of Health Research, 19(2), 6.
  10. Park, K. T., Jeon, Y. J., Kim, H. I., & Kim, W. (2023). Antinociceptive Effect of Dendrobii caulis in Paclitaxel-Induced Neuropathic Pain in Mice. Life, 13(12), 2289.
  11. Rafaqat, S., Gluscevic, S., Mercantepe, F., Rafaqat, S., & Klisic, A. (2024). Interleukins: Pathogenesis in Non-Alcoholic Fatty Liver Disease. Metabolites, 14(3), 153.
  12. Sahar Muayad Noori; Nadheerah Falih Neamah, a. S. A. A.-J. (2024). Study The Effects of 5 Selenocyantouracil Compound on The liver and Kidney of Male Rats After Acetaminophen-Induced Liver Injury Iraqi Journal of Pharmaceutical, 24(1). doi: https://doi.org/10.31351/vol33iss1pp23-32
  13. Salehi, B., Martorell, M., Arbiser, J. L., Sureda, A., Martins, N., Maurya, P. K., . . . Sharifi-Rad, J. (2018). Antioxidants: Positive or Negative Actors? Biomolecules, 8(4). doi: 10.3390/biom8040124
  14. Shiau, M.-Y., Chuang, P.-H., Yang, C.-P., Hsiao, C.-W., Chang, S.-W., Chang, K.-Y., . . . Chang, Y.-H. (2019). Mechanism of Interleukin-4 Reducing Lipid Deposit by Regulating Hormone-Sensitive Lipase. Sci Rep, 9(1), 11974. doi: 10.1038/s41598-019-47908-9
  15. Steinke, J. W., & Borish, L. (2001). Th2 cytokines and asthma — Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists. Respiratory Research, 2(2), 66. doi: 10.1186/rr40
  16. Suleyman, H., Albayrak, A., Bilici, M., Cadirci, E., & Halici, Z. (2010). Different mechanisms in formation and prevention of indomethacin-induced gastric ulcers. Inflammation, 33(4), 224-234. doi: 10.1007/s10753-009-9176-5
  17. Suparmi, S., Fasitasari, M., Martosupono, M., & Mangimbulude, J. C. (2016). Comparisons of Curative Effects of Chlorophyll from Sauropus androgynus (L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats. Journal of Toxicology, 2016, 8515089. doi: 10.1155/2016/8515089
  18. Tagauov, Y. D., Abu-Elsaoud, A. M., Abdrassulova, Z. T., Tuleukhanov, S. T., Salybekova, N. N., Tulindinova, G., & Al-Abkal, F. (2023). Improvement of Blood Parameters of Male Rats Exposed to Different Injection Doses of Liquid Chlorophyll. Cureus, 15(3), e36044. doi: 10.7759/cureus.36044
  19. Zhu, F., Du, B., & Xu, B. (2018). Anti-inflammatory effects of phytochemicals from fruits, vegetables, and food legumes: A review. Crit Rev Food Sci Nutr, 58(8), 1260-1270. doi: 10.1080/10408398.2016.1251390