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Academia Open

Microbiology

Vol 9 No 2 (2024): December

Enhanced Protease Production from Diabetic Ulcer-Derived E. coli
Peningkatan Produksi Protease dari E. coli yang Berasal dari Ulkus Diabetes



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.9.2024.9142
Published
June 18, 2024

Abstract

This study explored a local Escherichia coli isolate from diabetic foot ulcer patients for protease enzyme production. Qualitative screening on skim milk agar showed a 6 mm hydrolysis halo, while quantitative screening with casein revealed a specific activity of 144.55 U/mg protein. Purification via ion exchange chromatography on DEAE-cellulose increased the specific activity to 767.5 U/mg, with a 5.3-fold enhancement and a 29.5% yield. The findings suggest E. coli as a viable source for protease production and demonstrate the efficiency of ion-exchange chromatography for enzyme purification, with potential significant industrial applications.

Highlight:

  1. E. coli sourced from diabetic ulcers shows promise for protease production.
  2. Skim milk agar and casein used for qualitative and quantitative screening.
  3. Ion exchange chromatography significantly enhances enzyme specific activity.

 

Keywoard: Escherichia coli, protease production, diabetic foot ulcer, enzyme purification, ion exchange chromatography

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