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Academia Open

Microbiology

Vol 8 No 2 (2023): December

Isolation and Preparation Three Types of Antigens From S.pseudintermedius
Isolasi dan Persiapan Tiga Jenis Antigen dari S. pseudintermedius.



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.8.2023.7158
Published
August 8, 2023

Abstract

This study aimed to prepare and evaluate the immunogenic activity of three distinct antigens derived from S. pseudintermedius, a prominent isolate identified from otitis externa samples of dogs and human infections. The isolation and identification of Staphylococcus species were conducted using rigorous cultural, morphological, and biochemical methods, with confirmation via VITEK technique. A total of 100 samples, comprising 50 from humans and 50 from dogs, were collected. Following successful antigen preparation, twenty rabbits were immunized and divided into four groups, including a control group. Cellular immunity was assessed through a delayed hypersensitivity (DTH) skin test, revealing positive skin reactions among the immunized groups. Subsequent challenge dosing resulted in clinical observation, with marked signs of illness and severe infection in the control group. Histopathological examination further confirmed this infection and bacterial isolation. Remarkably, the prepared antigens exhibited substantial protein concentrations and induced a robust immune response in immunized rabbits against experimental L. monocytogenes infection. These findings underscore the potential of S. pseudintermedius antigens for developing protective strategies against bacterial infections in both veterinary and human medicine, offering significant implications for global health practitioners and researchers in the field.

Highlights :

  • Rigorous identification: S. pseudintermedius derived antigens evaluated after precise Staphylococcus species identification using cultural, morphological, and biochemical methods, confirmed via VITEK technique.
  • Robust immune response: Immunization with prepared antigens induced positive delayed hypersensitivity skin reactions and protected against severe L. monocytogenes infection, demonstrating potential for cross-species bacterial infection prevention.
  • Clinical and histopathological insights: Control group exhibited illness and Listeriosis upon challenge, validated through histopathological examination, highlighting antigen-induced protection against bacterial invasion.

References

  1. E. A. Al-Hasnawi, "Isolation of Staphylococcus aureus from ear swab in Iraqi children as a causative agent of Otitis externa," Journal of the Faculty of Medicine Baghdad, vol. 59, no. 3, pp. 258-261, 2017.
  2. H. S. Jasim, "Antibiotic Resistance of Staphylococcus aureus isolated from nasal cavity of Health Care Personnel," Journal of the Faculty of Medicine Baghdad, vol. 54, no. 4, pp. 344-348, 2012.
  3. C. N. Krute and J. L. Bose, "Understanding staphylococcal nomenclature," in The Genetic Manipulation of Staphylococci, New York, NY: Humana Press, 2015, pp. 1-7.
  4. V. Vrbovská et al., "Characterization of Staphylococcus intermedius group isolates associated with animals from Antarctica and emended description of Staphylococcus delphini," Microorganisms, vol. 8, no. 2, p. 204, 2020.
  5. D. Osmonov et al., "Cardiac device-related endocarditis. 31-years’ experience," J. Cardiol., vol. 61, pp. 175–180, 2013.
  6. N. H. Hussein, "Prevalence and antimicrobial susceptibility patterns of bacteria isolated from urinary tract infections (UTIs) in children at children hospital in Baghdad," Al-Kindy College Medical Journal, vol. 13, no. 1, pp. 102-107, 2017.
  7. A. J. Ahmed, "Evaluation of the bactericidal effect of Nisin and/or Potassium sorbate and Sodium chloride on the viability of Staphylococcus aureus in soft cheese: Zina Saab Khudhir and Adnan Jawad Ahmed," The Iraqi Journal of Veterinary Medicine, vol. 41, no. 1, pp. 55-59, 2017.
  8. O. M. Radostits et al., "A textbook of the diseases of cattle, horses, sheep, pigs and goats," Veterinary medicine, vol. 10, pp. 2045-2050, 2007.
  9. A. H. Ali, "Using Punica l. Shell Extract As A bacteriostatic For Staphylococcus aureus And Inhancing Locally Processed Sausage: Ali H. Ali, Faris A. Al-Obaidi, Meiad G. Baqir, Shahrazad M. Al-Shadeedi," The Iraqi Journal of Veterinary Medicine, vol. 31, no. 2, pp. 61-69, 2007.
  10. M. H. G. Kanaan and A. H. A. AL-Shammary, "Detection of methicillin or multidrug resistant Staphylococcus aureus (MRSA) in locally produced raw milk and soft cheese in Baghdad markets," The Iraqi Journal of Veterinary Medicine, vol. 37, no. 2, pp. 226-231, 2013.
  11. J. Wyckoff, "Vet. Med. Team Targets Brucellosis," Internet, 2002.
  12. S. M. Hayyawi, "Comparison of microbial isolates isolated from external ear canal of sheep and their susceptibility to antibiotics," The Iraqi Journal of Veterinary Medicine, vol. 36, no. 0E, pp. 41-48, 2012.
  13. H. Y. Yassin, A. K. Melconian, and S. S. Mahmood, "PREVALENCE OF EXFOLIATIVE TOXIN GENES AMONG CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS IN IRAQ," Iraqi Journal of Agricultural Sciences, vol. 53, no. 2, pp. 465-470, 2022.
  14. A. A. Tahi, S. Sousa, K. Madani, C. L. Silva, and F. A. Miller, "Ultrasound and heat treatment effects on Staphylococcus aureus cell viability in orange juice," Ultrasonics Sonochemistry, vol. 78, p. 105743, 2021.
  15. Z. I. Ibrahim, "Study the influence of some Listeria monocytogenes antigens on the side effects of Mitomycin C," The Iraqi Journal of Veterinary Medicine, vol. 36, no. 0E, 2012.
  16. V. Ziamko and V. Okulich, "Method of isolation of peptidoglycan as a basis for measuring murein-destroying activity of blood serum," J Microbiol Exp, vol. 1, pp. 144-146, 2014.
  17. E. R. Al-dafai, "Using of Whole Sonicated, Peptidoglycan and Modified Method of Peptidoglycan Antigens of Staph aureus Agianst infection with the same Bacteria," M.Sc. Thesis in Veterinary Medicine, University of Baghdad, Baghdad-Iraq, 2013.
  18. R. Calderón-González et al., "Cellular vaccines in listeriosis: role of the Listeria antigen GAPDH," Frontiers in cellular and infection microbiology, vol. 4, p. 22, 2014.
  19. Hudson and F. C. Hay, "Practical Immunology," 3rd Ed. Black-Well Scientific Publication, Oxford London, 1980.
  20. L. G. Luna, "Manual of Histologic Staining Methods of the Armed Force Institute of Pathology," 3rd Ed. McGraw-Hill, New York, 1968.
  21. T. J. Kindt, R. A. Goldsby, and B. A. Osborne, "Kuby Immunology," 6th Ed. Library of Congress, Publisher, S. Tenney, W.H. Freeman and Company, Printing and Binding, R.R. Donnelley, New York, USA, 2007.
  22. D. John and C. T. Macarther, "Decrease in Cell Mediated Immunity a Marker for Allosteric Load Effect on Immune Function," Research Network on Socioeconomic Status and Health, 1997.
  23. R. R. Dietert, T. L. Bunn, and J. E. Lee, "The delayed type hypersensitivity assay using protein and xenogeneic cell antigens," in Immunotoxicity Testing: Methods and Protocols, 185-194, 2010.
  24. I. R. Tizard, "Veterinary Immunology-E-Book," Elsevier Health Sciences, 2017.
  25. C. P. Subhash, "Microbiology and Immunology Textbook of 2nd Edition," 2012.
  26. I. R. Tizard, "Veterinary Immunology an Introduction," 8th Ed. Saunders Elsevier, pp. 255-380, 2009.
  27. Z. Bercovich, E. A. ter Laak, and H. H. Van Lipzigj, "Detection of brucellosis in dairy herds after an outbreak of the disease using a delayed-type hypersensitivity test," Prev. Vet. Med., vol. 13, pp. 277-285, 1995.
  28. D. B. Davis, R. Dulbecco, N. H. Eisen, and S. H. Ginsborg, "Microbiology," 4th Ed., Lippincott Company, pp. 539-549, 1990.
  29. H. Moshtaghi, M. Arabi, and I. Karimi, "Histopathology and pathogenesis of listeriosis caused by Listeria monocytogenes isolated from raw milk in mice," Pakistan. J. Biol. Sci, vol. 9, pp. 2181-83, 2006.
  30. C. Rouquette and P. Berche, "The pathogenesis of infection by Listeria monocytogenes," Microbiologia (Madrid, Spain), vol. 12, no. 2, pp. 245-258, 1996.
  31. T. Mainou-Fowler, A. P. MacGowan, and R. Postlethwaite, "Virulence of Listeria spp.: course of infection in resistant and susceptible mice," Journal of medical microbiology, vol. 27, no. 2, pp. 131-140, 1988.
  32. V. Kumar, R. M. Mitchell, A. K. Abbas, and N. Fausto, "Basic Pathology," 8th edition, Pathology.bsd.uchicago.edu/faculty/V Kumar.html, 2009.
  33. E. B. Hawryluk, L. Izikson, and J. C. English, "Non-infectious granulomatous diseases of the skin and their associated systemic diseases," American journal of clinical dermatology, vol. 11, no. 3, pp. 171-181, 2010.
  34. B. M. Luna et al., "Vaccines targeting Staphylococcus aureus skin and bloodstream infections require different composition," PloS one, vol. 14, no. 6, p. e0217439, 2019.
  35. M. A. Cassatella, "The production of cytokines by polymorph nuclear neutrophils," Immunol. Immunol. Today, vol. 16, pp. 21-25, 1995.
  36. M. G. Netea et al., "Increased susceptibility of tumor necrosis factor – α lymphotoxin –alpha double knockout mice to systemic candidiasis through impaired recruitment of neutrophils and phagocytosis of Candida albicans," J. Immuno., vol. 163, pp. 1498-1505, 1999.
  37. T. L. McGaha and M. C. Karlsson, "Apoptotic cell responses in the splenic marginal zone: a paradigm for immunologic reactions to apoptotic antigens with implications for autoimmunity," Immunological reviews, vol. 269, no. 1, pp. 26-43, 2016.
  38. K. Matsuno, T. Ezaki, and M. Kotani, "Splenic outer periarterial lymphoid sheath (PALS): an immunoproliferative microenvironment constituted by antigen-laden marginal metallophils and ED2-positive macrophages in the rat," Cell and tissue research, vol. 257, no. 3, pp. 459-470, 1989.

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