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Academia Open

Clinical Research

Vol 8 No 2 (2023): December

Toxicity Testing of Turi Putih Bark Extract on Mice Liver
Pengujian Toksisitas Ekstrak Kulit Batang Turi Putih pada Hati Mencit



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.8.2023.5429
Published
October 25, 2024

Abstract

General background the use of natural extracts in assessing toxicity has gained attention due to the growing interest in alternative medicine and natural products. Specific background Sesbania grandiflora, commonly known as turi putih, is a plant with potential medicinal properties, but its safety profile regarding hepatic toxicity remains inadequately explored. Knowledge gap while previous studies have examined various plants for their pharmacological effects, research specifically focusing on the hepatotoxicity of turi putih extract is lacking. Aims this study aims to evaluate the toxicity of ethanol extract from the bark of Sesbania grandiflora on liver function, as measured by SGOT and SGPT levels, in Mus musculus. Results showed no mortality or toxic symptoms in the mice exposed to the extract. Macroscopic examination of the liver indicated normal color, consistency, and shape. Statistical analysis yielded p-values of 0.502 for liver weight, 0.226 for SGOT, and 0.366 for SGPT, indicating no significant effects from the treatment. Novelty this research contributes new insights into the safety profile of Sesbania grandiflora, demonstrating its non-toxic effects at the tested doses. Implications the findings suggest that the bark extract of turi putih may be safe for use, highlighting its potential in herbal medicine without adverse hepatic effects.

Highlights: 

  • No observed toxicity or mortality in mice after exposure to the extract.
  • Normal liver function indicated by SGOT and SGPT levels.
  • Potential safety of turi putih bark extract for medicinal use.

Keywords: Turi Putih, Hepatotoxicity, Sesbania grandiflora, Mice, Ethanol Extract

References

  1. A. K. Makalalag, M. Sangi, and M. Kuamunang, “Skrining fitokimia dan uji toksisitas ekstrak etanol dari daun turi (Sesbania grandiflora (L.) Pers),” ChemProg, vol. 8, no. 1, 2015. [Online]. Available: https://ejournal.unsrat.ac.id/index.php/chemprog/article/view/9442
  2. N. Yuliyan, “Isolasi dan identifikasi senyawa fenolik dari kulit batang tumbuhan turi putih (Sesbania grandiflora, L) serta uji aktivitas antibakteri,” B.Sc. thesis, Universitas Lampung, Bandar Lampung, Indonesia, 2018. [Online]. Available: https://eprints.umm.ac.id/70123/1/PENDAHULUAN.pdf
  3. F. Resanty, “Pengaruh perendaman ekstrak air daun turi (Sesbania grandiflora (L.) Pers.) terhadap regenerasi sirip kaudal ikan zebra (Danio rerio),” B.Sc. thesis, Universitas Islam Indonesia, Yogyakarta, Indonesia, 2020. [Online]. Available: https://dspace.uii.ac.id/bitstream/handle/123456789/28782/16613061%20Fety%20Resanty.pdf?sequence=1&isAllowed=y
  4. J. Rohmah, I. A. Saidi, C. S. Rini, D. A. Masyitha, D. N. Ramadhani, and H. P. Wulandari, “Aktivitas antioksidan ekstrak etanol, etil asetat, dan n-heksana batang turi putih (Sesbania grandiflora (L.) Pers.) dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl),” Jurnal Kimia Riset, vol. 5, pp. 67–85, 2020. [Online]. Available: http://dx.doi.org/10.20473/jkr.v5i1.20900
  5. S. Sumayya, “Uji toksisitas akut ekstrak air daun turi (Sesbania grandiflora, L) pada embrio ikan zebra (Danio rerio),” B.Sc. thesis, Universitas Islam Indonesia, Yogyakarta, Indonesia, 2019. [Online]. Available: https://dspace.uii.ac.id/handle/123456789/17000?show=full
  6. H. Widenfeld, “Plants containing pyrrolizidine alkaloids: toxicity and problems,” Crit. Rev. Toxicol., vol. 41, no. 4, pp. 327–337, 2011. [Online]. Available: https://doi.org/10.1080/19440049.2010.541288
  7. H. S. Wicaksono, I. Narayani, and I. Setyawati, “Struktur hati mencit (Mus musculus L.) setelah pemberian ekstrak daun kaliandra merah (Calliandra calothyrsus Meissn.),” Jurnal Simbiosis III, vol. 1, pp. 258–268, 2015. [Online]. Available: https://ojs.unud.ac.id/index.php/simbiosis/article/download/14405/9901
  8. A. K. Syam, “Uji toksisitas akut ekstrak etanol daun kayu hitam (Diospyros celebica B.) terhadap mencit (Mus musculus),” B.Sc. thesis, Universitas Islam Negeri Alauddin Makassar, Samata-Gowa, Indonesia, 2016. [Online]. Available: http://repositori.uin-alauddin.ac.id/1347/1/ASWIN%20KHALIQ%20SYAM%20SKRIPSI.PDF
  9. J. V. Mustapa, “Pengaruh perubahan kadar flavonoid pada penyimpanan ekstrak etanol daun turi (Sesbania grandiflora) terhadap potensinya sebagai insektida lalat hijau (Chrysomya megacephala) melalui metode semprot,” B.Sc. thesis, Universitas Brawijaya, Malang, Indonesia, 2018. [Online]. Available: http://repository.ub.ac.id/167971/1/Junaidi%20Virgiawan%20Mustafa.pdf
  10. S. Fajariyah, E. T. Utami, and Y. Arisandi, “Efek pemberian estrogen sintetis (Diethylstillbestrol) terhadap struktur hepar dan kadar SGOT dan SGPT pada mencit (Mus musculus) betina strain Balb’C,” Jurnal Ilmu Dasar, vol. 11, no. 1, 2010. [Online]. Available: http://journal.unej.ac.id/index.php/ilmu-dasar/article/view/1543
  11. W. Amananti, I. Tivani, and B. A. Riyanta, “Uji kandungan saponin pada daun, tangkai daun dan biji tanaman turi (Sesbania grandiflora),” in Seminar Nasional IPTEK Terapan (SENIT), 2017, pp. 209–213.
  12. A. N. F. Ahmadita, “Formulasi losion ekstrak etanol 70% herba kemangi (Ocimum americanum L.) menggunakan asam stearat sebagai emulgator,” B.Sc. thesis, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Islam Negeri Syarif Hidayatullah Jakarta, 2017. [Online]. Available: https://www.onesearch.id/Record/IOS3796.123456789-36735/TOC
  13. D. A. Sari, “Daya antioksidan ekstrak metanol batang turi putih (Sesbania grandiflora (L.) Pers.) dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl),” B.Sc. thesis, Program Studi D-IV Teknologi Laboratorium Medis Fakultas Ilmu Kesehatan Universitas Muhammadiyah Sidoarjo, 2019.
  14. H. P. Wulandari, “Aktivitas antioksidan ekstrak etanol batang turi putih (Sesbania grandiflora (L.) Pers.) dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl),” B.Sc. thesis, Universitas Muhammadiyah Sidoarjo, Sidoarjo, Indonesia, 2020.
  15. M. Masriani and F. S. Budi, “Penapisan fitokimia ekstrak metanol beberapa tumbuhan obat asal Kalimantan Barat,” Jurnal Ilmu Pengetahuan Alam dan Teknologi, vol. 5, no. 2, 2017. [Online]. Available: https://repository.untan.ac.id/index.php?p=show_detail&id=3606&keywords=
  16. A. M. Jannah, "Uji Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Daun Salam (Syzygium Polyanthum) Hasil Sonikasi dengan Variasi Pelarut," Skripsi, Universitas Islam Negeri Maulana Malik Ibrahim Malang, 2021. [Online]. Available: http://etheses.uin-malang.ac.id/32860/.
  17. A. I. Habibi, R. A. Firmansyah, and S. M. Setyawati, "Skrining Fitokimia Ekstrak n-Heksan Korteks Batang Salam (Syzygium Polyanthum)," Indonesian Journal of Chemical Science, vol. 7, no. 1, 2018. [Online]. Available: https://www.semanticscholar.org/paper/Skrining-Fitokimia-Ekstrak-n-Heksan-Korteks-Batang-Habibi-Firmansyah/bd367faad86489ef4d6a909bba8f9f159937c7a4.
  18. N. Ergina, S. Nuryanti, and I. D. Pursitasari, "Uji Kualitatif Senyawa Metabolit Sekunder pada Daun Palado (Agave Angustifolia) yang Diekstraksi dengan Pelarut Air dan Etanol," Jurnal Ilmiah, vol. 3, no. 3, pp. 165-172, 2014. [Online]. Available: https://www.neliti.com/id/publications/224213/uji-kualitatif-senyawa-metabolit-sekunder-pada-daun-palado-agave-angustifolia-ya.
  19. S. Sihombing, "Uji Toksistas In Vivo Akut Ekstrak Etanol Daun Punguntano (Picria Fel-Terrae) pada Mencit Jantan," Skripsi, Universitas Sumatra Utara, Medan, 2017. [Online]. Available: http://etheses.uin-malang.ac.id/1019/1/04520042%20Skripsi.pdf.
  20. Chairani, "Gambaran Histologi dan Fungsi Hati (SGPT dan SGOT) Mencit Jantan (Mus Musculus L.) Setelah Pemberian Ekstrak Methanol Biji Pare (Momordica Charantia L.) dan Depo Medroksi Progesteron Asetat (DMPA)," Skripsi, Universitas Sumatra Utara, Medan, 2017. [Online]. Available: https://repositori.usu.ac.id/bitstream/handle/123456789/3006/130805037.pdf?sequence=1&isAllowed=y.
  21. T. R. A. Wijayanti, "Pengaruh Pemberian Ekstrak Daun Turi Merah Terhadap Jumlah Makrofag pada Mencit Nifas yang Diinfeksi Streptococcus Agalactiae," Jurnal Kesehatan Hesti Wira Sakti, vol. 5, no. 1, pp. 56-59, 2017. [Online]. Available: https://jurnal.poltekkes-soepraoen.ac.id/index.php/HWS/article/view/156.
  22. C. D. K. Wati, "Uji Toksisitas Subakut Infusa Biji Persea Americana Mill pada Tikus Galur Sprague Dawley Terhadap Kadar Blood Urea Nitrogen dan Kreatinin," Skripsi, Fakultas Farmasi Universitas Sanata Dharma, Yogyakarta, 2015. [Online]. Available: https://123dok.com/document/q7w40rrz-toksisitas-subakut-persea-americana-sprague-terhadap-nitrogen-kreatinin.html.
  23. L. M. Kurniawidjaja, F. Lestari, M. Tejamaya, and D. H. Ramdhan, "Konsep Dasar Toksikologi Industri," Fakultas Kesehatan Masyarakat Universitas Indonesia, 2021.
  24. O. P. Windiarko, "Pengaruh Preventif Madu Hutan Sumbawa Terhadap Gambaran Histopatologi Hepar, Kadar SGPT, dan SGOT Darah Tikus (Rattus Norvegicus) yang Diinduksi Plumbum Asetat," Skripsi, Universitas Brawijaya, 2018. [Online]. Available: http://repository.ub.ac.id/id/eprint/14138/.

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