Academia Open
Vol 10 No 1 (2025): June (In Progress)
Medicine

Exploring the Role of Insulin Resistance in Hormonal imbalance in PCOS patients
Menjelajahi Peran Resistensi Insulin dalam Ketidakseimbangan Hormon pada Pasien PCOS


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  • Nabil Hamdullah Al-Fahadi*,
  • Aya dhaher J,
  • Astbraq Haitham A.,
  • Sidra Yassin H.
Nabil Hamdullah Al-Fahadi
University of Al-Hadbaa, Iraq *
Aya dhaher J
University of Al-Hadbaa, Iraq
Astbraq Haitham A.
University of Al-Hadbaa, Iraq
Sidra Yassin H.
University of Al-Hadbaa, Iraq

(*) Corresponding Author
Picture in here are illustration from public domain image or provided by the author, as part of their works
DOI: https://doi.org/10.21070/acopen.10.2025.10765
Published March 15, 2025
Keywords
  • Urinary Tract Infection,
  • Beta-Thalassemia,
  • Virulence Factors,
  • Bacterial Pathogens,
  • 16S rRNA Sequencing
How to Cite
Al-Fahadi, N. H., J, A. dhaher, A., A. H., & H., S. Y. (2025). Exploring the Role of Insulin Resistance in Hormonal imbalance in PCOS patients. Academia Open, 10(1), 10.21070/acopen.10.2025.10765. https://doi.org/10.21070/acopen.10.2025.10765

Copyright (c) 2025 Nabil Hamdullah Al-Fahadi, Aya dhaher J, Astbraq Haitham A., Sidra Yassin H.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

General Background: Urinary tract infections (UTIs) are a significant concern for transfusion-dependent beta-thalassemia (TDT) patients due to their compromised immune systems and frequent hospital visits. Specific Background: Identifying virulence factor genes in bacterial species isolated from UTIs in TDT patients is crucial for understanding pathogenicity and improving treatment strategies. Knowledge Gap: Despite the high prevalence of UTIs in TDT patients, limited studies have focused on detecting virulence factor genes in the bacterial isolates from this specific population. Aims: This study aims to identify virulence factor genes in bacteria isolated from the urine samples of TDT patients with UTIs. Results: Among 173 urine samples, bacterial growth was observed in 38 samples (21.96%), while 135 samples (79.03%) showed no growth. The identified isolates included Escherichia coli, Enterobacter cloacae, and Klebsiella pneumoniae, confirmed through DNA extraction, universal primers, and partial 16S rRNA sequencing. Novelty: This study provides new insights into the molecular characteristics of bacterial pathogens in TDT patients, highlighting the presence of specific virulence factors that contribute to infection severity. Implications: The findings enhance our understanding of bacterial virulence in TDT-related UTIs, offering a foundation for targeted therapeutic strategies and improved clinical management.

Highlights:

 

  1. UTI Risk: TDT patients have higher susceptibility due to immune dysfunction.
  2. Bacterial Profile: E. coli, E. cloacae, and K. pneumoniae dominate infections.
  3. Clinical Impact: Supports targeted therapy for managing UTIs in TDT patients.

 

Keywords: Urinary Tract Infection, Beta-Thalassemia, Virulence Factors, Bacterial Pathogens, 16S rRNA Sequencing

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