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Exploring the Role of Insulin Resistance in Hormonal imbalance in PCOS patients

Menjelajahi Peran Resistensi Insulin dalam Ketidakseimbangan Hormon pada Pasien PCOS Section Medicine
Vol 10 No 1 (2025): June (In Progress):
DOI : https://doi.org/10.21070/acopen.10.2025.10765
Published : Mar 15, 2025

Nabil Hamdullah Al-Fahadi (1), Aya dhaher J (2), Astbraq Haitham A. (3), Sidra Yassin H. (4)

(1) University of Al-Hadbaa, Iraq
(2) University of Al-Hadbaa, Iraq
(3) University of Al-Hadbaa, Iraq
(4) University of Al-Hadbaa, Iraq
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Abstract:

General Background: Urinary tract infections (UTIs) are a significant concern for transfusion-dependent beta-thalassemia (TDT) patients due to their compromised immune systems and frequent hospital visits. Specific Background: Identifying virulence factor genes in bacterial species isolated from UTIs in TDT patients is crucial for understanding pathogenicity and improving treatment strategies. Knowledge Gap: Despite the high prevalence of UTIs in TDT patients, limited studies have focused on detecting virulence factor genes in the bacterial isolates from this specific population. Aims: This study aims to identify virulence factor genes in bacteria isolated from the urine samples of TDT patients with UTIs. Results: Among 173 urine samples, bacterial growth was observed in 38 samples (21.96%), while 135 samples (79.03%) showed no growth. The identified isolates included Escherichia coli, Enterobacter cloacae, and Klebsiella pneumoniae, confirmed through DNA extraction, universal primers, and partial 16S rRNA sequencing. Novelty: This study provides new insights into the molecular characteristics of bacterial pathogens in TDT patients, highlighting the presence of specific virulence factors that contribute to infection severity. Implications: The findings enhance our understanding of bacterial virulence in TDT-related UTIs, offering a foundation for targeted therapeutic strategies and improved clinical management.


Highlights:


 



  1. UTI Risk: TDT patients have higher susceptibility due to immune dysfunction.

  2. Bacterial Profile: E. coli, E. cloacae, and K. pneumoniae dominate infections.

  3. Clinical Impact: Supports targeted therapy for managing UTIs in TDT patients.


 


Keywords: Urinary Tract Infection, Beta-Thalassemia, Virulence Factors, Bacterial Pathogens, 16S rRNA Sequencing

References

References:

A. Smith, B. Johnson, “Prevalence of Polycystic Ovary Syndrome and Its Impact on Reproductive Health,” J. of Reproductive Medicine, vol. 12, no. 3, pp. 32-40, 2019.

C. Lee, D. Brown, “Metabolic Dysfunction and Insulin Resistance in PCOS: Mechanisms and Therapeutic Approaches,” Endocrinology Reviews, vol. 44, no. 2, pp. 128-135, 2020.

R. Patel, “Hormonal Imbalances in PCOS and Their Role in Infertility,” Reprod. Biol. Endocrinol., vol. 18, no. 1, pp. 45-52, 2021.

L. Wright, M. Kim, “Hyperandrogenism and Its Clinical Manifestations in PCOS,” J. Clin. Endocrinol. Metab., vol. 102, no. 7, pp. 1115-1123, 2017.

S. Gupta, T. Sharma, “Insulin Resistance and Metabolic Risk Factors in PCOS,” Diabetes Care, vol. 44, no. 9, pp. 238-245, 2021.

H. Zhang, L. Liu, “Insulin Resistance in Women with PCOS: Prevalence, Pathophysiology, and Treatment,” J. of Obstetrics and Gynaecology, vol. 32, no. 6, pp. 782-790, 2022.

M. Singh, P. Verma, “Association Between Insulin Resistance and Hormonal Changes in PCOS Patients,” Endocrinol. Metab. Clin. North Am., vol. 47, no. 4, pp. 529-537, 2018.

R. Miller, “The Rotterdam Criteria for PCOS: A Comprehensive Review,” J. Fertil. Reprod., vol. 54, no. 1, pp. 100-110, 2020.

K. Brown, “Hyperprolactinemia and Its Effect on PCOS,” J. Endocrinological Investigations, vol. 29, no. 3, pp. 85-92, 2020.

J. Thomas, “Long-Term Cardiovascular Risk in Women with PCOS,” J. Cardiovascular Res., vol. 28, no. 4, pp. 500-508, 2019.

T. Walker, D. Moore, “Lean PCOS: Insulin Resistance in Non-Obese Women,” J. of Clinical Endocrinology, vol. 38, no. 7, pp. 214-221, 2022.

N. Patel, S. Raghav, “Metformin Treatment and Its Impact on Insulin Sensitivity in PCOS,” J. Obstetrics and Gynaecology, vol. 43, no. 5, pp. 1092-1099, 2021.

P. Lee, “Genetic Factors Involved in PCOS and Insulin Resistance,” Genetics in Medicine, vol. 35, no. 2, pp. 223-230, 2022.

L. Ramirez, “Endocrine Disruptors and Their Effect on PCOS Pathophysiology,” J. Environ. Health, vol. 27, no. 3, pp. 145-151, 2021.

R. Jones, A. Moore, “Effects of Insulin Resistance on Ovarian Function in PCOS,” J. Endocrinol., Metab., and Reprod., vol. 53, no. 6, pp. 1102-1111, 2018.

C. Kato, “PCOS and Its Association with Cardiovascular Diseases,” J. Cardiovasc. Dis. Prev., vol. 13, no. 4, pp. 58-65, 2019.

M. Davis, “Clinical Manifestations of Insulin Resistance in Women with PCOS,” Intern. J. Endocrinol., Diab. Metab., vol. 32, no. 5, pp. 34-39, 2021.

F. Reed, “Pathogenesis of Hyperandrogenism in Women with PCOS,” J. Women’s Health, vol. 25, no. 3, pp. 200-210, 2022.

P. Fisher, “Metabolic Effects of Hyperinsulinemia in PCOS Patients,” Endocrinology Research, vol. 14, no. 2, pp. 110-115, 2020.

J. Patel, M. Lee, “Insulin Resistance and the Development of Type 2 Diabetes in Women with PCOS,” J. Diabetic Care, vol. 18, no. 8, pp. 213-220, 2022.

K. Singh, “Obesity and Its Impact on Hormonal Regulation in PCOS,” J. Obesity Studies, vol. 21, no. 4, pp. 270-275, 2021.

S. Williams, “Role of the Liver in Insulin Resistance in Women with PCOS,” J. Endocrinol. Metab., vol. 55, no. 2, pp. 116-123, 2021.

N. Torres, “Obesity and Inflammation in Women with PCOS,” Diabetes and Endocrinol. J., vol. 43, no. 9, pp. 48-54, 2022.

M. Schmidt, “Impact of Lifestyle Modifications on PCOS and Insulin Sensitivity,” J. of Nutritional Science, vol. 45, no. 3, pp. 215-220, 2020.