Abstract:
General Background: Osteoporosis, a common condition exacerbated by glucocorticoid use, leads to significant bone loss and increased fracture risk. Specific Background: Conventional treatments primarily target calcium regulation, but emerging evidence suggests that natural compounds like isoflavonoids may offer therapeutic benefits. Knowledge Gap: Despite their potential, the efficacy of isoflavonoids and their nanoformulations in managing glucocorticoid-induced osteoporosis remains underexplored. Aims: This study aimed to evaluate the therapeutic effects of vitamin D, Ginkgo biloba isoflavonoid extract, and nanoisoflavonoids on glucocorticoid-induced osteoporosis in adult male rats. Results: Sixty rats were divided into five groups: a control, an osteoporosis model, and three treatment groups receiving vitamin D (10,000 IU/day), isoflavonoid extract (500 mg/kg/day), or nanoisoflavonoids (500 mg/kg/day) for one month. Molecular and physiological assessments revealed that nanoisoflavonoids were the most effective in restoring serum vitamin D levels, reducing parathyroid hormone concentrations, and downregulating parathyroid hormone gene expression, followed by isoflavonoid extract and vitamin D. Novelty: This study provides the first comprehensive evaluation of nanoisoflavonoids' superior efficacy in mitigating glucocorticoid-induced osteoporosis, highlighting their enhanced bioavailability and therapeutic potential. Implications: These findings suggest that nanoisoflavonoids could serve as a promising plant-derived nanotherapeutic for osteoporosis management, warranting further investigation through long-term studies and clinical trials to validate their safety and efficacy in human populations.
Highlights:
- Glucocorticoid-induced osteoporosis lacks effective natural compound treatments.
- Nanoisoflavonoids restore vitamin D, reduce parathyroid hormone effectively.
- Nanoisoflavonoids offer a promising therapeutic strategy for osteoporosis management.
Keywords: Osteoporosis, glucocorticoids, Ginkgo biloba, isoflavonoids, nanoisoflavonoids, vitamin D, parathyroid hormone, bone metabolism
References
References
A. R. Sabeeh and M. H. Al-Saeed, "Evaluation the role of supplementation of Vit. D on some physiological parameters, semen quality in hypovitaminosis D male rabbits induced by furosemide," Basrah Journal of Veterinary Research, vol. 19, no. 2, pp. 259-280, Sep. 2020.
S. H. Alwan and M. H. Al-Saeed, "Silver nanoparticles biofabricated from Cinnamomum zeylanicum reduce IL-6, IL-18, and TNF-ɑ in female rats with polycystic ovarian syndrome," International Journal of Fertility & Sterility, vol. 17, no. 1, pp. 1-14, Jan.–Mar. 2023.
W. A. Jawad and M. H. Al-Saeed, "Evaluation of the effect of flavonoid extract of Ginkgo biloba leaves and Glimephan on oxidative stress and retina degeneration in diabetic male rabbits induced by streptozotocin," Basrah Journal of Veterinary Research, vol. 19, no. 1, pp. 182-205, Oct. 2019.
S. H. Alwan, M. H. Al-Saeed, and H. A. Abid, "Safety assessment and biochemical evaluation of the effect of biogenic silver nanoparticles (using bark extract of C. zeylanicum) on Rattus norvegicus rats," Baghdad Journal of Biochemistry and Applied Biological Sciences, vol. 2, no. 3, pp. 133-145, Jul. 2021.
S. H. Alwan and M. H. Al-Saeed, "Biosynthesized silver nanoparticles (using Cinnamomum zeylanicum bark extract) improve the fertility status of rats with polycystic ovarian syndrome," Biocatalysis and Agricultural Biotechnology, vol. 38, article 102217, pp. 1-14, Nov. 2021.
J. Burch, J. Harwood, and J. Hodge, "The effects of glucocorticoids on bone metabolism: Implications for osteoporosis," Journal of Bone Metabolism, vol. 27, no. 1, pp. 1-11, 2020, doi: 10.11005/jbm.2020.27.1.1.
J. Fang, X. Zhang, and Y. Li, "Bioactive flavonoids: A review of their role in bone health," Critical Reviews in Food Science and Nutrition, vol. 59, no. 18, pp. 2959-2970, 2019, doi: 10.1080/10408398.2018.1458709.
S. Kumar, R. Gupta, and R. Joshi, "Role of isoflavonoids in osteogenic differentiation: A review," Current Osteoporosis Reports, vol. 19, no. 1, pp. 35-45, 2021, doi: 10.1007/s11914-021-00627-8.
C. Li, Y. Chen, and Z. Wu, "Protective effects of Ginkgo biloba extract on bone loss: A review," Phytotherapy Research, vol. 32, no. 5, pp. 797-805, 2018, doi: 10.1002/ptr.6107.
M. Thakur, S. Sahu, and A. Kumar, "Green synthesis of nanoparticles from plant extracts: A review on current trends and applications," Materials Today: Proceedings, vol. 45, pp. 3713-39, 2021, doi: 10.1016/j.matpr.2021.05.336.
]11 [D. Yadav, R. Kumar, and A. Singh, “Ginkgo biloba-derived nanoparticles: A new perspective in drug delivery and therapeutics,” Int. J. Nanomedicine, vol. 17, pp. 253–267, 2022. [Online]. Available: https://doi.org/10.2147/IJN.S348493
]12 [ J. Zhang, L. Zhang, and C. Huang, “Effects of isoflavonoids on bone metabolism: A potential strategy for osteoporosis treatment,” Front. Pharmacol., vol. 11, p. 217, 2020. [Online]. Available: https://doi.org/10.3389/fphar.2020.00217
]13 [ Y. Liu, H. Sun, and Z. Zhang, “The role of vitamin D in bone health: A comprehensive review,” Front. Nutr., vol. 9, Article 810672, 2022. [Online]. Available: https://doi.org/10.3389/fnut.2022.810672
]14[ S. Pilz, A. Tomaschitz, and C. L. Wagner, “Vitamin D and osteoporosis: A review of the current literature,” Endocrine, vol. 74, no. 3, pp. 579–588, 2021. [Online]. Available: https://doi.org/10.1007/s12020-021-02764-3
]15[ M. Sahni and R. R. McLean, “Vitamin D and bone health: A critical review,” Bone Rep., vol. 15, Article 101069, 2021. [Online]. Available: https://doi.org/10.1016/j.bonr.2021.101069
]16[ Q. Geng et al., “A soluble bone morphogenetic protein type 1A receptor fusion protein treatment prevents glucocorticoid-induced bone loss in mice,” 2019. [Online]. Available: https://pmc.ncbi.nlm.nih.gov/articles/PMC6684880/
]17[ L. W. B. Lamb et al., “Permitted daily exposure from preclinical studies of Ginkgo biloba L. dry extract,” Braz. J. Pharm. Sci., vol. 59, 2023. [Online]. Available: https://doi.org/10.1590/s2175-97902023e23037
]18[ Y. Ren, H. Yang, T. Wang, and C. Wang, “Green synthesis and antimicrobial activity of monodisperse silver nanoparticles synthesized using Ginkgo Biloba leaf extract,” Phys. Lett. A, vol. 380, no. 45, pp. 3773–3777, 2016. [Online]. Available: https://doi.org/10.1016/j.physleta.2016.09.029
]19[ M. E. Rodriguez-Ortiz et al., “Magnesium modulates parathyroid hormone secretion and upregulates parathyroid receptor expression at moderately low calcium concentration,” Nephrol. Dial. Transplant., vol. 29, no. 2, pp. 282–289, 2013. [Online]. Available: https://doi.org/10.1093/ndt/gft400