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Academia Open

Clinical Research

Vol 9 No 2 (2024): December

The Effects of Crude Alkaloid Extracts of Convolutes Arvensis on Tumour Cell Lines and Their Potential For Toxicity
Efek Ekstrak Alkaloid Kasar dari Convolutes Arvensis pada Garis Sel Tumor dan Potensi Toksisitasnya



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.9.2024.10314
Published
October 17, 2024

Abstract

General Background: Cancer remains a leading cause of mortality worldwide, and the search for effective treatments continues to intensify. Specific Background: Convolvulus arvensis has been traditionally used for medicinal purposes, but its potential as an anticancer agent is underexplored. Knowledge Gap: The cytotoxic and apoptotic mechanisms of crude alkaloids extracted from C. arvensis against specific cancer cell lines have not been fully characterized. Aims: This study investigates the antioxidant activity and cytotoxic effects of C. arvensis crude alkaloids on mouse liver cancer (HC) and human breast cancer (AMJ13) cell lines, focusing on apoptosis-related gene expression. Results: The antioxidant activity of C. arvensis was comparable to that of ascorbic acid, with inhibition rates of 92.01% at 500 µg/ml. Crude alkaloids demonstrated dose-dependent cytotoxicity, with a maximum inhibition rate of 82.65% in AMJ13 cells and 79.49% in HC cells at 500 µg/ml. Gene expression analysis revealed upregulation of caspase-9, indicating apoptosis via the intrinsic mitochondrial pathway. Novelty: This study is among the first to provide molecular evidence of C. arvensis-induced apoptosis through the intrinsic pathway, offering a novel insight into its anticancer potential. Implications: These findings suggest that C. arvensis alkaloids could be developed as a therapeutic option for cancer treatment, with future studies needed to isolate specific compounds and assess their in vivo efficacy.

Highlights:

 

  1. C. arvensis alkaloids show antioxidant activity similar to ascorbic acid.
  2. Alkaloids inhibit liver and breast cancer cell growth dose-dependently.
  3. Apoptosis triggered via caspase-9 through the mitochondrial pathway.

 

Keywords: Convolvulus arvensis, alkaloids, cancer, apoptosis, antioxidant

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