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Academia Open

Microbiology

Vol 9 No 2 (2024): December

Removal of Toxic Heavy Metals Using Genetically Engineered Microbes: Molecular Tools, Risk Assessment, and Management Strategies
Penghapusan Logam Berat Beracun Menggunakan Mikroba Hasil Rekayasa Genetika: Alat Molekuler, Penilaian Risiko, dan Strategi Manajemen



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.9.2024.10300
Published
October 15, 2024

Abstract

Background: The growing occurrence of heavy metal pollutants in many environmental sources requires effective methods for treatment. Genetically engineered microorganisms, namely bacteria such as Shewanella oneidensis and Cupriavidus metallidurans, have been highly useful for the specific removal of heavy metals. Purpose: This study aims to examine the effectiveness of genetically modified Shewanella oneidensis and Cupriavidus metallidurans, obtained from MB Genetics, in removing harmful heavy metals from various environmental sources. Methods: Genetically modified strains were obtained from MB Genetics, a company specializing in the creation of transgenic microbes. Shewanella oneidensis and Cupriavidus metallidurans were utilized to mitigate the presence of harmful heavy metals in water under different pH levels. Results: The study showed a notable effectiveness, as both bacterial strains successfully eliminated 91% of Lead at pH 7. The study highlighted the substantial impact of pH on the levels of heavy metals in the environment. Conclusion: The need to eradicate harmful heavy metals in the present time can be efficiently tackled by using genetically modified bacteria. Shewanella oneidensis and Cupriavidus metallidurans demonstrated remarkable efficacy in the removal of heavy metals.

Highlights:

 

  1. Modified bacteria remove 91% of lead at neutral pH.
  2. CRISPR/Cas9 enhances bacteria for efficient heavy metal removal.
  3. pH optimization is key for effective heavy metal absorption.

 

Keywords: Toxic heavy metals, Pseudomonas aeruginosa, Shewanella oneidensis, Cupriavidus metallidurans

References

  1. . F. Akram, S. Sahreen, F. Aamir, I. U. Haq, K. Malik, M. Imtiaz, et al., “An Insight into Modern Targeted Genome-Editing Technologies with a Special Focus on CRISPR/Cas9 and its Applications,” Molecular Biotechnology, vol. 65, no. 2, pp. 227–242, 2023.
  2. . K. H. H. Aziz, F. S. Mustafa, K. M. Omer, S. Hama, R. F. Hamarawf, and K. O. Rahman, “Heavy Metal Pollution in the Aquatic Environment: Efficient and Low-Cost Removal Approaches to Eliminate Their Toxicity: A Review,” RSC Advances, vol. 13, no. 26, pp. 17595–17610, 2023.
  3. . A. Bhatt, J. Shandilya, S. K. Singal, and S. K. Prajapati, “Application of Genetically Modified Microorganisms for Bioremediation of Heavy Metals from Wastewater,” in Genomics Approach to Bioremediation: Principles, Tools, and Emerging Technologies, 1st ed., Cham: Springer Nature, 2023, pp. 295–320.
  4. . A. M. M. Chatha, S. Naz, S. S. Iqbal, and U. Zahra, “Investigating the Impact of Environmental Toxicology of Heavy Metals in Fish: A Study of Rivers of Pakistan,” Scientific Inquiry and Review, vol. 7, no. 4, pp. 67–90, 2023.
  5. . S. Chen and Y. Ding, “A Bibliography Study of Shewanella oneidensis Biofilm,” FEMS Microbiology Ecology, vol. 99, no. 11, article fiad124, 2023.
  6. . R. Dhanker, T. Hussain, P. Tyagi, K. J. Singh, and S. S. Kamble, “The Emerging Trend of Bio-Engineering Approaches for Microbial Nanomaterial Synthesis and Its Applications,” Frontiers in Microbiology, vol. 12, article 638003, 2021.
  7. . P. Diep, R. Mahadevan, and A. F. Yakunin, “Heavy Metal Removal by Bioaccumulation Using Genetically Engineered Microorganisms,” Frontiers in Bioengineering and Biotechnology, vol. 6, article 157, 2018.
  8. . R. S. Dongre, “Lead: Toxicological Profile, Pollution Aspects and Remedial Solutions,” in Lead Chemistry, 1st ed., Cham: Springer Nature, 2020, pp. 1–8.
  9. . EFSA Scientific Committee, S. More, V. Bampidis, D. Benford, C. Bragard, T. Halldorsson, et al., “Evaluation of Existing Guidelines for Their Adequacy for the Microbial Characterisation and Environmental Risk Assessment of Microorganisms Obtained Through Synthetic Biology,” EFSA Journal, vol. 18, no. 10, article e06263, 2020.
  10. . N. Guha, S. Walke, and P. Thiagarajan, “Microbial Strategies to Address Environmental Nanopollutants,” in Relationship Between Microbes and the Environment for Sustainable Ecosystem Services, Volume 2, 1st ed., Amsterdam: Elsevier, 2022, pp. 151–179.
  11. . N. Gupta, M. Singh, P. Gupta, P. Mishra, and V. Gupta, “Heavy Metal Bioremediation and Toxicity Removal from Industrial Wastewater,” in Next-Generation Algae: Volume I: Applications in Agriculture, Food, and Environment, Cham: Springer Nature, 2023, pp. 163–193.
  12. . A. I. Hassan and H. M. Saleh, “Toxins and Pollutants Detection on Biosensor Surfaces,” in Toxic Gas Sensors and Biosensors, 1st ed., Cham: Springer Nature, 2021, pp. 197–219.
  13. . A. Hassanien, I. Saadaoui, K. Schipper, S. Al-Marri, T. Dalgamouni, M. Aouida, et al., “Genetic Engineering to Enhance Microalgal-Based Produced Water Treatment with Emphasis on CRISPR/Cas9: A Review,” Frontiers in Bioengineering and Biotechnology, vol. 10, article 1104914, 2023.
  14. . Q. Huang, B. Lin, Y. Cao, Y. Zhang, H. Song, C. Huang, et al., “CRISPR/Cas9-Mediated Mutagenesis of the Susceptibility Gene OsHPP04 in Rice Confers Enhanced Resistance to Rice Root-Knot Nematode,” Frontiers in Plant Science, vol. 14, article 1134653, 2023.
  15. . N. I. M. Ibrahim, “The Relations Between Concentration of Iron and the pH Ground Water (Case Study Zulfi Ground Water),” International Journal of Environmental Monitoring and Analysis, vol. 4, no. 6, pp. 140–145, 2016.
  16. . J. K. Jansson, R. McClure, and R. G. Egbert, “Soil Microbiome Engineering for Sustainability in a Changing Environment,” Nature Biotechnology, pp. 1–13, 2023.
  17. . N. Johnson, “Revolutionizing Soil Remediation: Exploring the Frontiers of Bioremediation Through the Performance Evaluation of Vernonia Galamensis and Vernonia Amydalina Spices in Hydrocarbon-Contaminated Soil,” Environmental Biotechnology, vol. 12, no. 2, pp. 44-57, 2024.
  18. . S. Joshi, S. Gangola, G. Bhandari, N. S. Bhandari, D. Nainwal, A. Rani, et al., “Rhizospheric Bacteria: The Key to Sustainable Heavy Metal Detoxification Strategies,” Frontiers in Microbiology, vol. 14, article 746293, 2023.
  19. . H. Karimi-Maleh, M. Ghalkhani, Z. S. Dehkordi, J. Singh, Y. Wen, M. Baghayeri, et al., “MOF-Enabled Pesticides as a Developing Approach for Sustainable Agriculture and Reducing Environmental Hazards,” Journal of Industrial and Engineering Chemistry, vol. 106, pp. 50–65, 2023.
  20. . R. Kumar, L. Singh, A. W. Zularisam, and F. I. Hai, “Microbial Fuel Cell is Emerging as a Versatile Technology: A Review on its Possible Applications, Challenges and Strategies to Improve the Performances,” International Journal of Energy Research, vol. 42, no. 2, pp. 369–394, 2018.
  21. . C. Liu, H. Yu, B. Zhang, S. Liu, C. G. Liu, F. Li, and H. Song, “Engineering Whole-Cell Microbial Biosensors: Design Principles and Applications in Monitoring and Treatment of Heavy Metals and Organic Pollutants,” Biotechnology Advances, article 108019, 2022.
  22. . X. Li, L. Zhang, Z. Yang, P. Wang, Y. Yan, and J. Ran, “Adsorption Materials for Volatile Organic Compounds (VOCs) and the Key Factors for VOCs Adsorption Process: A Review,” Separation and Purification Technology, vol. 235, article 116213, 2020.
  23. . M. Medfu Tarekegn, F. Zewdu Salilih, and A. I. Ishetu, “Microbes Used as a Tool for Bioremediation of Heavy Metal from the Environment,” Cogent Food & Agriculture, vol. 6, no. 1, article 1783174, 2020.
  24. . A. Monga, A. B. Fulke, M. D. Giripunje, and D. Dasgupta, “Microbial Remediation Technologies for Chromium Removal: Mechanism, Challenges and Future Prospect,” in Chromium in Plants and Environment, 1st ed., Cham: Springer Nature, 2023, pp. 319–384.
  25. . T. V. Plavec and A. Berlec, “Safety Aspects of Genetically Modified Lactic Acid Bacteria,” Microorganisms, vol. 8, no. 2, article 297, 2020.
  26. . A. K. Priya, L. Gnanasekaran, K. Dutta, S. Rajendran, D. Balakrishnan, and M. Soto-Moscoso, “Biosorption of Heavy Metals by Microorganisms: Evaluation of Different Underlying Mechanisms,” Chemosphere, vol. 307, article 135957, 2022.
  27. . N. A. Qasem, R. H. Mohammed, and D. U. Lawal, “Removal of Heavy Metal Ions from Wastewater: A Comprehensive and Critical Review,” NPJ Clean Water, vol. 4, no. 1, article 36, 2021.
  28. . M. K. Sharma, S. Kumar, and B. Nag, “The Role of Plasmid-Encoded Genes in Heavy Metal and Antibiotic Resistance in Enterobacteriaceae Species,” Journal of Applied Microbiology, vol. 133, no. 1, pp. 72–85, 2023.
  29. . A. Singh and A. Chaturvedi, “Genomic Approaches and Microbial Interactions in Bioremediation: An Overview,” in Microbial Genomics in Sustainable Agroecosystems: Volume 2: Microbial Interactions and Bioremediation, Cham: Springer Nature, 2022, pp. 77–112.
  30. . M. Swaranlata, G. Tarun, and K. Prabha, “Wastewater Treatment Using Microbial Biofilms: Current Trends, Challenges, and Future Perspectives,” Frontiers in Microbiology, vol. 13, article 889475, 2022.
  31. . K. S. Sharma, K. Panchal, M. Chhimwal, and D. Kumar, “Integrated Detection and Natural Remediation Technology as a Low-Cost Alternative for Wastewater Treatment,” Health Sciences Review, vol. 8, article 100111, 2023.
  32. . P. Sharma, A. Bano, S. P. Singh, S. Sharma, C. Xia, A. K. Nadda, et al., “Engineered Microbes as Effective Tools for the Remediation of Polyaromatic Hydrocarbons and Heavy Metals,” Chemosphere, vol. 306, article 135538, 2022.
  33. . P. Sharma, S. P. Singh, H. M. Iqbal, R. Parra-Saldivar, S. Varjani, and Y. W. Tong, “Genetic Modifications Associated with Sustainability Aspects for Sustainable Developments,” Bioengineered, vol. 13, no. 4, pp. 9509–9521, 2022.
  34. . S. Singh and V. Kumar, “Mercury Detoxification by Absorption, Mercuric Ion Reductase, and Exopolysaccharides: A Comprehensive Study,” Environmental Science and Pollution Research, vol. 27, no. 22, pp. 27181–27201, 2020.
  35. . J. F. Su, Y. C. Gao, T. L. Huang, X. C. Bai, J. S. Lu, and L. He, “Simultaneous Removal of Cd2+, NO3-N and Hardness by the Bacterium Acinetobacter sp. CN86 in Aerobic Conditions,” Bioprocess and Biosystems Engineering, vol. 42, pp. 1333–1342, 2019.
  36. . Z. Syed, M. Sogani, J. Rajvanshi, and K. Sonu, “Microbial Biofilms for Environmental Bioremediation of Heavy Metals: A Review,” Applied Biochemistry and Biotechnology, vol. 195, no. 9, pp. 5693–5711, 2023.
  37. . T. D. Thai, W. Lim, and D. Na, “Synthetic Bacteria for the Detection and Bioremediation of Heavy Metals,” Frontiers in Bioengineering and Biotechnology, vol. 11, article 1178680, 2023.
  38. . U. J. Contreras and C. M. Gardner, “Environmental Fate and Behaviour of Antibiotic Resistance Genes and Small Interference RNAs Released from Genetically Modified Crops,” Journal of Applied Microbiology, vol. 133, no. 5, pp. 2877–2892, 2022.
  39. . S. Verma, P. Bhatt, A. Verma, H. Mudila, P. Prasher, and E. R. Rene, “Microbial Technologies for Heavy Metal Remediation: Effect of Process Conditions and Current Practices,” Clean Technologies and Environmental Policy, pp. 1–23, 2021.
  40. . Y. Wang, V. Selvamani, I. K. Yoo, T. W. Kim, and S. H. Hong, “A Novel Strategy for the Microbial Removal of Heavy Metals: Cell-Surface Display of Peptides,” Biotechnology and Bioprocess Engineering, vol. 26, pp. 1–9, 2021.
  41. . C. Wu, F. Li, S. Yi, and F. Ge, “Genetically Engineered Microbial Remediation of Soils Co-Contaminated by Heavy Metals and Polycyclic Aromatic Hydrocarbons: Advances and Ecological Risk Assessment,” Journal of Environmental Management, vol. 296, article 113185, 2021.
  42. . A. A. Yaqoob, M. N. M. Ibrahim, S. Rodríguez-Couto, and A. Ahmad, “Preparation, Characterization, and Application of Modified Carbonized Lignin as an Anode for Sustainable Microbial Fuel Cell,” Process Safety and Environmental Protection, vol. 155, pp. 49–60, 2021.
  43. . M. Zaynab, R. Al-Yahyai, A. Ameen, Y. Sharif, L. Ali, M. Fatima, et al., “Health and Environmental Effects of Heavy Metals,” Journal of King Saud University - Science, vol. 34, no. 1, article 101653, 2022.
  44. . B. Zhou, T. Zhang, and F. Wang, “Microbial-Based Heavy Metal Bioremediation: Toxicity and Eco-Friendly Approaches to Heavy Metal Decontamination,” Applied Sciences, vol. 13, no. 14, article 8439, 2023.
  45. . J. Zou, X. Liu, D. Zhang, and X. Yuan, “Adsorption of Three Bivalent Metals by Four Chemically Distinct Microplastics,” Chemosphere, vol. 248, article 126064, 2020.

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