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Academia Open

Clinical Research

Vol 9 No 2 (2024): December

Flavonoids from Verbascum thapsus Protect Against Nephrotoxicity in Rats
Flavonoid dari Verbascum thapsus Melindungi Terhadap Nefrotoksisitas pada Tikus



(*) Corresponding Author
DOI
https://doi.org/10.21070/acopen.9.2024.10298
Published
October 14, 2024

Abstract

General Background: Nephrotoxicity induced by carbon tetrachloride (CCl4) poses significant health risks, prompting the exploration of natural antioxidants for renal protection. Specific Background: Verbascum thapsus, a plant known for its flavonoid content, has shown potential in mitigating oxidative stress, but its specific protective effects against CCl4-induced nephrotoxicity remain under-investigated. Knowledge Gap: While previous studies have indicated antioxidant properties of flavonoids, their efficacy in renal preservation in vivo has not been fully elucidated. Aims: The study evaluated the antioxidative and nephroprotective properties of flavonoids from Verbascum thapsus leaves in a rat model of CCl4-induced nephrotoxicity.Results: Rats were divided into three groups: control, CCl4-treated, and flavonoid plus CCl4-treated. Biochemical analyses revealed that CCl4 significantly elevated malondialdehyde (MDA), urea, creatinine, and uric acid levels while decreasing superoxide dismutase (SOD) and glutathione (GSH) levels. Notably, flavonoid administration markedly reduced MDA, urea, creatinine, and uric acid levels and enhanced SOD and GSH levels compared to the CCl4-only group. Novelty: This study uniquely highlights the protective role of flavonoids from Verbascum thapsus against nephrotoxicity, demonstrating both their antioxidative capacity and renal preservation in an experimental model. Implications: The findings support the potential use of Verbascum thapsus as a therapeutic agent in the management of kidney diseases, suggesting further investigation into its application in clinical settings to address nephrotoxicity and related renal disorders.

Highlights:

 

  1. Nephroprotective: Flavonoids significantly protect against CCl4-induced kidney damage.
  2. Biochemical Enhancement: Improved antioxidant levels; reduced harmful biochemical indicators.
  3. Therapeutic Potential: Supports natural treatments for kidney diseases.

 

Keywords: Verbascum thapsus, flavonoids, nephrotoxicity, antioxidants, carbon tetrachloride

References

  1. . O. B. Adewale and N. E. Orhue, "Protective Effect of Aqueous Extract of Xylopia Aethiopica Fruits on Carbon Tetrachloride-Induced Nephrotoxicity in Rats," J. Exp. Integr. Med., vol. 5, no. 2, pp. 105–109, 2015.
  2. . S. Bhattacharya, "Reactive Oxygen Species and Cellular Defense System," in Free Radicals in Human Health and Disease, Springer, 2015, pp. 17–29.
  3. . K. Daenen, A. Andries, D. Mekahli, A. Van Schepdael, F. Jouret, and B. Bammens, "Oxidative Stress in Chronic Kidney Disease," Pediatr. Nephrol., vol. 34, pp. 975–991, 2019.
  4. . D. Lian, M. Chen, H. Wu, S. Deng, and X. Hu, "The Role of Oxidative Stress in Skeletal Muscle Myogenesis and Muscle Disease," Antioxidants, vol. 11, no. 4, p. 755, 2022.
  5. . H. J. Forman and H. Zhang, "Targeting Oxidative Stress in Disease: Promise and Limitations of Antioxidant Therapy," Nat. Rev. Drug Discov., vol. 20, pp. 689–709, 2021.
  6. . H. Yoshioka, H. Usuda, N. Fukuishi, T. Nonogaki, and S. Onosaka, "Carbon Tetrachloride-Induced Nephrotoxicity in Mice Is Prevented by Pretreatment with Zinc Sulfate," Biol. Pharm. Bull., vol. 39, no. 6, pp. 1042–1046, 2016.
  7. . S. E. M. Ali and D. H. A. Abdelaziz, "The Protective Effect of Date Seeds on Nephrotoxicity Induced by Carbon Tetrachloride in Rats," Int. J. Pharm. Sci. Rev. Res., vol. 26, no. 2, pp. 62–68, 2014.
  8. . F. H. Kamisan, F. Yahya, S. S. Mamat, and M. F. F. Kamarolzaman, "Effect of Methanol Extract of Dicranopteris Linearis Against Carbon Tetrachloride-Induced Acute Liver Injury in Rats," BMC Complement. Altern. Med., vol. 14, no. 1, p. 123, 2014.
  9. . M. A. Hamed, S. A. Ali, and N. S. El-Rigal, "Therapeutic Potential of Ginger Against Renal Injury Induced by Carbon Tetrachloride in Rats," Sci. World J., vol. 2012, p. 12, 2012.
  10. . V. Waisundara, Antioxidants: Benefits, Sources, Mechanisms of Action, 2021.
  11. . A. Zeb, "Concept, Mechanism, and Applications of Phenolic Antioxidants in Foods," Food Biochem., vol. 44, no. 9, 2020.
  12. . I. I. Tatli and Z. S. Akdemir, "Traditional Uses and Biological Activities of Verbascum Species," Fabad J. Pharm. Sci., vol. 31, pp. 85–96, 2006.
  13. . L. Speranza et al., "Anti-Inflammatory Effects in THP-1 Cells Treated with Verbascoside," Phytother. Res., vol. 24, pp. 1398–1404, 2010.
  14. . T. Remaley, "Common Mullein (Verbascum Thapsus)," Plant Conservation Alliance, Alien Plant Working Group, National Park Service, 2005.
  15. . V. Prakash and A. Sagar, "Investigation of Leaf Extracts of Verbascum Thapsus Linn. for Their Antioxidant Potential," J. Med. Plants Stud., vol. 9, no. 1, pp. 37–40, 2021.
  16. . B. I. Harborn, Phytochemical Methods: A Guide to Modern Technology of Plant Analysis, 2nd ed. New York: Chapman and Hall, 1984, pp. 88–185.
  17. . S. Yadav and P. Kumar, "Production, Isolation, and Identification of Flavonoids from Aerial Parts of Hiptage Benghalensis," Int. J. Life Sci. Pharma Res., vol. 2, no. 3, pp. 5, 2012.
  18. . S. K. Yadav and A. V. Trivedi, "Determination of LD50 Value of Zinc Chloride on Swiss Albino Mice," Int. J. Curr. Microbiol. App. Sci., vol. 5, no. 5, pp. 910–913, 2016.
  19. . M. G. Palanivel, B. Rajkapoor, R. S. Kumar, and J. W. Einstein, "Hepatoprotective and Antioxidant Effect of Pisonia Aculeate L. Against CCl4-Induced Hepatic Damage in Rats," Sci. Pharm., vol. 76, no. 2, pp. 203–215, 2008.
  20. . M. S. Moron, J. W. Depierre, and B. Mannervik, "Levels of Glutathione, Glutathione Reductase, and Glutathione-S-Transferase Activities in Rat Lung and Liver," Biochim. Biophys. Acta, vol. 582, pp. 67–78, 1979.
  21. . J. Fawcett and J. A. Scott, "Rapid and Precise Method for the Determination of Urea," J. Clin. Pathol., vol. 13, pp. 156–159, 1960.
  22. . G. Szasz, U. Borner, E. W. Busch, and W. Bablok, "Enzymatic Assay of Creatinine in Serum: Comparison with Jaffe Methods," J. Clin. Chem. Clin. Biochem., vol. 17, pp. 683–687, 1979.
  23. . P. Fossati, L. Principe, and G. Berti, "Use of 3,5-Dichlorohydroxybenzenesulfonic Acid and 4-Aminophenazone Chromogenic System in Direct Enzymatic Assay of Uric Acid in Serum and Urine," Clin. Chem., vol. 26, pp. 227–231, 1980.
  24. . A. Abirami, G. Nagarani, and P. Siddhuraju, "Hepatoprotective Effect of Leaf Extracts from Citrus Hystrix and C. Maxima Against Paracetamol-Induced Liver Injury in Rats," Food Sci. Hum. Wellness, vol. 4, no. 1, pp. 35–41, 2015.
  25. . E. A. Hashish and S. A. Elgaml, "Hepatoprotective and Nephroprotective Effect of Curcumin Against Copper Toxicity in Rats," Indian J. Clin. Biochem., vol. 31, pp. 270–277, 2016.
  26. . S. Burki, Z. G. Burki, M. A. Asghar, I. Ali, and S. Zafar, "Phytochemical, Acute Toxicity and Renal Protective Appraisal of Ajuga Parviflora Hydromethanolic Leaf Extract Against CCl4 Induced Renal Injury in Rats," BMC Complement. Med. Ther., vol. 21, p. 198, 2021.
  27. . M. Hasanuzzaman, K. Nahar, T. I. Anee, and M. Fujita, "Glutathione in Plants: Biosynthesis and Physiological Role in Environmental Stress Tolerance," Physiol. Mol. Biol. Plants, vol. 23, pp. 249–268, 2017.
  28. . J. Pizzorno and I. Med, "Glutathione," Encinitas, vol. 13, no. 1, pp. 8–12, 2014.
  29. . R. Masella et al., "Novel Mechanisms of Natural Antioxidant Compounds in Biological Systems: Involvement of Glutathione and Glutathione-Related Enzymes," J. Nutr. Biochem., vol. 16, pp. 577–586, 2005.
  30. . M. R. Khan, W. Rizvi, G. N. Khan, R. A. Khan, and S. Shaheen, "Carbon Tetrachloride-Induced Nephrotoxicity in Rats: Protective Role of Digera Muricata," J. Ethnopharmacol., vol. 122, no. 1, pp. 91–99, 2009.
  31. . M. R. Khan and D. Ahmed, "Protective Effects of Digera Muricata (L.) Mart. on Testis Against Oxidative Stress of Carbon Tetrachloride in Rats," Food Chem. Toxicol., vol. 47, no. 6, pp. 1393–1399, 2009.
  32. . A. A. Gomaa et al., "Antioxidant, Hepatoprotective and Nephroprotective Activities of Gazania Rigens Against Carbon Tetrachloride-Induced Hepatotoxicity and Nephrotoxicity in Rats," Tradit. Med. Res., vol. 7, no. 5, pp. 44, 2022.
  33. . A. A. Rahmat, F. A. Dar, and I. M. Choudhary, "Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis Ferruginea (De Candolle)," Pharmacogn. Res., vol. 6, pp. 19–28, 2014.
  34. . S. S. Hanaa et al., "Nephroprotective Effect of Malva Sylvestris Extract Against CCl4 Induced Nephrotoxicity in Albino Rats," J. Food Dairy Sci., vol. 3, 3rd Mansoura International Food Congress (MIFC), pp. 37–43, 2018.

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