Comparative Analysis of Lutein Content in Leaves and Fruit of Lycium barbarum: Implications for Medicinal Applications Analisis Perbandingan Kandungan Lutein pada Daun dan Buah Lycium barbarum: Implikasi untuk Aplikasi Obat Zirjawi Omaima Saddam umaymasaddam@gmail.com Almousawi Ula M Noor umaymasaddam@gmail.com Khamees Amjed Haseeb umaymasaddam@gmail.com Iraq Iraq Iraq 17 10 2023 Introduction

Folk herbal medicine has been highly used over many decades all over the world alike, and this because its natural origin and its minimum side effects when compared with other synthetic drugs. plants characterized by it contain of therapeutically important constituents. Theis compounds are extracted from herbs and purified for its therapeutic benefit [1].

Lycium barbarum, also referred to as LB, Gouqi, wolfberry, or Fructus lycii, is a widely recognized traditional herbal plant that exhibits a broad geographical range. The significance of this substance in China and other Asian nations is of great importance, as it serves not only as a dietary supplement for daily use, but also possesses medicinal properties. L. barbarum, has garnered significant attention from both Chinese and international medical researchers and dietetic health professionals in recent years. This heightened interest can be attributed to the comprehensive examination of LB, which has revealed its noteworthy antioxidant properties and potential anti-aging benefits. [2]

L. barbarum is regarded as a botanical species with therapeutic properties that have been utilized in traditional and folk herbal medicine practices. The herbal raw material of Lycium, scientifically known as fructus Lycii and cortex Lycii radicis, encompasses its fruit and bark. However, it is worth noting that the therapeutic chemicals can also be found in the seeds and leaves of Lycium. The fruits of L. barbarum exhibit various therapeutic properties, including antiaging, protective, immunostimulant, energizing, adapt genic, anticancer, and antioxidant activity. These fruits are also abundant in biologically active compounds such as specific polysaccharides, carotenoids, flavonoids, terpenoids, vitamins B and C, and the element germanium.[3]

Carotenoids, which include oxygen-containing xanthophylls and oxygen-free carotenes, are present throughout the tree of life .a tiny set of carotenoids that are necessary for human nutrition and must be received from diet, such as lutein, zeaxanthin, and β-carotene (provitamin A)[4]

Lutein is a fat-soluble carotenoid pigment composed of 40 carbon atoms, including a sequence of prominent conjugated double bonds.

The conspicuous red color and propensity to supply free radicals in these substances are attributed to the presence of double bonds in their structure.[5]

Lutein (, -Carotene- 3, 3' diol) is an oxygenated derivative of hydrocarbon carotenoids that occurs naturally.It is found in different plants. It plays a physiological role in increasing eyesight and protecting the eyes from damaging UV rays. [6]

This study focus on lutein in the L.barbarum extract of leaves and fruit and compare between them when the extraction method and the solvent is the same

 Material and Methods:

1. Plant Material:

The plant was collected from different cities (Baghdad, Basrah, Maysan). then the specimen was diagnosed by Dr Ula Almosawi in the Pharmacognosy Laboratory, Faculty of Pharmacy at Basrah University.

It was first cleaned, washed and air dried at room temperature for 5 days. Then, the plant parts were crushed using blender until it become powder and prepared it to extraction process.

2. Extraction methods

To make the extracts, 5 grams of each plant part from each area were weighed out one by one, and 100 milliliters of 85% methanol were added to a 250-milliliter conical jar as a solvent. Our ultrasonic bath (DS-2510 DT) was set to 60 kHz and left on for 30 minutes at room temperature. All of the extracts were filtered and put in the fridge until they were analyzed [7]

3. High Performance Liquid Chromatography (HPLC) Analysis of lutein in Crude Extracts :

An HPLC analysis was performed for the detection and estimation of lutein in twelve extracts of plant. Analysis was carried out by HPLC system (Shimadzu) and the analysis was done in ministry of since and technology /Baghdad

HPLC Conditions:

Mobile phase :

A- Acetonitrile 76%

B- Methanol 21.5%

C- n- hexane 2.5%

Column type: ODS c18 (250 * 4.6 Id)mm, 5um particle size

Flow rate: 1ml/min

Temperature: at room temp.

Volume injection: 20ml

 Results and Discussion Stander

Figure and table HPLC of the lutein in the leaves extract of Baghdad

Figure and table HPLC of the lutein in the fruit extract of Baghdad

Figure and table HPLC of the lutein in the leaves extract of Basrah

Figure and table HPLC of the lutein in the fruit extract of Basrah

Figure and table HPLC of the lutein in the leaves extract of Maysan

Figure and table HPLC of the lutein in the fruit extract of Maysan

Were the dilution factor is 20

Area of the sample is the highest beak

Area of the stander= 3227187

The concentration of lutein

City Conc. Of Lutein in leaves Lutein in fruit
Baghdad 6.95mg/ml 1.7mg/ml
Basrah 10.88mg/ml 10.44mg/ml
Maysan 7.9mg/ml 4.1mg/ml
Conclusion

The experiment was done to measure the concentration of lutein in both leaves and fruit of L. barbarum and compare between concentration of lutein in leaves and fruit.

The result illustrate that both leaves and fruits extract contain lutein but in each region the leaves extract have lutein in higher concentration than fruit extract.

Our study shown that the lutein found in highly present in green leaves than fruit and this is match with Alisa Perry